Pramod P Wangikar

Location

136, Chemical Engineering

Contact Information

call

  • +91 (22) 2576 7232 (o)

Email

  • wangikar [at] iitb [dot] ac [dot] in

Pramod P Wangikar

Core Faculty

Professor

Background

  1. B. Chem. Eng. (University of Bombay 1991)

  2. Ph. D. (University of Iowa 1995)

Professional Appointments

  1. DBT-Pan IIT Center for Bioenergy IIT Bombay
  2. Wadhwani Research Center for Bioengineering (WRCB) IIT Bombay

Awards & Fellowships

  • National Bioscience Award for Career Development 2006 (DBT Government of India).

  • INAE Young Engineer Award 2005

  • G. R. Manudhane Excellence in Research Award (IIT Bombay) 2005

  • BOYSCAST fellowship (DST Govt of India) 2003

  • DAE Young Scientist Award (Dept. of Atomic Energy Govt. of India) 1997.

  • AICTE Career Award for Young Teachers 1998.

Enzyme Engineering for Biotransformation

13C Metabolic flux analysis of cyanobacteria. Our results show relatively low flux through the TCA cycle compared to Calvin cycle. Further, the photorespiration flux is negligible making this an efficient chassis for metabolic engineering. The flux values were obtained using dynamic labeling data of 17 metabolites.

Deployment of enzymes in industrial production of chemicals requires development on three fronts: (i) Design of enzymes with desired char- acteristics of activity, selectivity, stability, substrate tolerance, etc., (ii) Cost effective production of the enzyme and (iii) Development of bio-transformation process. Wangikar lab’s current efforts are on all three fronts with focus on developing processes for chiral synthesis using two classes of enzymes; nitrilase and alcohol dehydrogenase (ADH). De-sign of enzymes is initiated with experimental testing of unexplored sequences of putative enzymes followed by directed evolution of prom-ising candidates. We also undertake rational, model based design of en-zymes in collaboration with other groups that have expertise in protein X-raycrystallography. Currently, the group has designed novel ADH enzymes that show >99% stereoselectivity and > 100 units activity / ml of fermentation broth. We envisage the use of whole cell biocatalyst for cost effective chiral synthesis. We have also designed acofactor recycle system with a recycle ratio of 1:2,000.

Algal CO 2 capture and utilization (CCU)

Metabolic oscillations in cyanobacteria. Profiles of (A) CO2  and (B) O2  in the exit gas (cyan), pH of the growth medium (brown), (C) intracellular glycogen (red) content and growth (green) in the fourth day of entrainment under alternating light/dark cycles (LD) followed by constant light (LL). The horizontal bar below the X-axis denotes the light (clear), dark (shaded) and subjective dark (shaded with slanted lines) phases.

Atmospheric CO 2 levels have been rising at alarming rates over the last two centuries, and are projected to reach 700-1000 ppm (parts per mil-lion) by year 2100. Carbon and energy accounting reveals that a typical microalgal CCU is far from being net carbon negative or net energy positive, primarily because of high operational energy requirements. Further, even a medium sized Thermal Power Station would require algal ponds of over hundred square kilometers, thereby posing the challenge of capital and scale. Wangikar’s group works towards design of algal strains and processes to improve the aerial productivity. Their strain selection criteria include: (i) volumetric and aerial productivity, (ii) bio-mass concentration at the end of batch, (iii) tolerance to local climatic conditions, (iv) tolerance to CO 2 and other flue gas components, and (v) ability to synthesize storage molecules. Algal strains isolated from
the plant site of our industry partner fulfill many of these requirements and are now ready to be tested for CO 2 capture at the plant site. Apart from this, Wangikar’s group also works on metabolic engineering of cyanobacteria for photoautotrophic production of high value compounds. This includes physiological characterization, genome sequencing, metabolic model construction, flux analysis and several other systems biology approaches. We have performed 13C flux analysis on model strains of cyanobacteria with current efforts on local strains. Further, we have identified a number of cyanobacterial promoters that are under the control of an internal circadian clock.