Start
Oct 09, 2015 - 14:30
End
Oct 09, 2015 - 15:30
Venue
Room # 118 Chem. Engg. Dept.
Event Type
Speaker
S.Subhashree Ph.D. Scholar Dept of Biochemistry and Molecular Biology Pondicherry University.
Title
Studies on the neuroprotective effect of Valeriana wallichii rhizome extract in in vitro and in vivo models of Parkinson's disease .
Abstract: Parkinson’s disease (PD) is a chronic progressive movement disorder due to loss of dopaminergic neurons in substantia nigra pars compacta region of mid brain.The present study was carried out to evaluate the protective effect of Valeriana wallichii rhizome extract against MPP+ treated in vitro and MPTP treated in vivo models of Parkinson’s disease. Qualitative analysis of the aqueous ethanolic and methanolic extracts of V. wallichii rhizome were carried out which indicated the presence of flavonoids phenols glycosides terpinoids and tannins.HPTLC quantification showed that the methanolic extract of V. wallichii rhizome has highest amount of hesperidin. Subsequent studies were carried out with the methanolic extract of V. wallichii rhizome (VWE). Analysis of VWE by GC-MS speculated the presence of acacetin valeric acid homovannelic acid etc. VWE showed in vitro radical scavenging activities reductive ability.VWE exhibited IC 50 value at 2.207 mg/mL for SH-SY5Y cells. In SH-SY5Y neuroblastoma cells treated with neurotoxin MPP+ or ER stress inducer tunicamycin time and dose dependent effect of VWE was analysed. VWE at a concentration of 1000µg/mL protected the SH-SY5Y cells. As evidenced by morphological staining VWE exhibited ROS scavenging activity prevented apoptotic cell death and inhibited mitochondrial. For in vivo experiments C57BL/6 male mice treated with sub chronic level of MPTP were used as PD model. Following MPTP treatment behavioural studies were performed. PD mice were treated orally with 3 different doses of VWE for 2 weeks. The mid brain regions/striatum was used for analysing various parameters and for histopathological analysis. VWE improved the behavioral test scores increased the striatal dopamine mid brain tyrosine hydroxylase levels reduced GFAP ROS LPO levels MAO-B activity and increased the GSH levels antioxidant enzyme activities in a dose dependent manner in PD mice. Also VWE restrained the MPTP induced histopathological changes in mid brain region of PD mice. Optimum dose fixed from the results observed was used for further studies. VWE effectively reduced the inflammatory markers regulated the apoptotic and ER stress markers. In silico molecular docking studies of selective components of VWE with MAO-B iNOS and PERK showed that acacetin interacted better with MAO-B whereas hesperidin exhibited best binding efficiency with iNOS and PERK. Data obtained in this study indicates the potential of VWE to reduce MPTP induced PD. While this may be attributed to the synergistic effects of various phytocompounds present in it the potential of hesperidin on important selective markers emphasize its therapeutic value. Thus Valeriana wallichii and its bioactive compounds can serve as potential leads for the development of several new drugs for treating PD.Bio-data: S.Subhashree is a Ph.D. Scholar of Dept. of Biochemistry and Molecular Biology in Pondicherry University.