Start
Sep 25, 2014 - 17:00
End
Sep 25, 2014 - 18:00
Venue
Creativity Hall Room 118 Chemical Engineering
Event Type
Speaker
Dr. Ashish Misra DBT Energy Bioscience Overseas Fellow Institute of Chemical Technology Bombay.
Title
Deducing Cellular Carbon Traffic Patterns – Applications of Metabolic Analyses
Abstract: Metabolic analyses allow the delineation of properties of a metabolic network that govern cellular behaviour in response to specific cues. They include flux analyses for determining in vivo fluxes and in silico pathway analyses. In this talk I will discuss applications of some of these methodologies toward providing insights into cellular behaviour and suggesting modifications for improving cells toward a desired objective. Initially the use of combined flux and pathway analyses for studying an engineered yeast strain that produces a drug precursor will be shown. The use of labelling experiments for deducing in vivo fluxes in the engineered strain; and suggested improvements in the production of the drug precursor by the use of in silico analyses will be discussed. Then a detailed study of cells from poplar a model tree organism using labelled substrates will be shown. The utility of labelling experiments combined with detailed metabolic modelling to elucidate metabolite recycling in poplar cells will be demonstrated. Further an investigation of cellular behaviour in response to differing carbon and nitrogen availability using metabolic flux and gene expression analysis will be shown. These investigations have revealed interesting changes in intracellular metabolism and possible mechanisms for nitrogen usage by poplar which will be discussed. Finally the use of a previously developed dynamic modeling approach in analyzing the metabolic responses of a butanol producing microbe will be shown. The changes in fluxes at different stages of fermentations and relative contributions of specific elementary modes will bediscussed.About the Speaker: Ashish completed his degree in B. Chem. Eng. from the Institute of Chemical Technology (ICT) Mumbai in 2002. He received his M.S. in Chemical Engineering and Ph.D. in Chemical Engineering in 2005 and 2009 respectively from Rutgers The State University of New Jersey. His doctoral research focused on technique development for the analysis of genetic variations using affinity separation and mass spectrometry. He was a postdoctoral research associate at the University of Maryland from 2010 to 2012 on an NSF funded project and worked in the area of metabolic analyses. Since 2013 he has been working as a DBT Energy Bioscience Overseas Fellow at ICT in the area of metabolic analyses and engineering.
Attendance is mandatory for CL702 and 704