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The intracellular reaction rate analysis can provide a system level phenotypic endpoint for biological systems. This however cannot be extracted from the static measurements of individual protein or metabolite concentrations. Figure 1. Representative data to show 13C isotopic enrichment in 3-phosphoglycerate. Flux analysis requires metabolic model based interpretation of dynamic patterns of 13C-isotopic labelling of […]

The lab has developed fed-batch, high cell density cultivation protocols for the production of soluble proteins in E. coli BL21 (DE3). This fed-batch strategy is based on the reported high cell density protocols for E. coli with modifications to significantly improve soluble protein production. Representative profile for the dissolved oxygen and substrate feed in a […]

Alcohol dehydrogenase (ADH) catalyzed asymmetric reduction is a powerful approach to synthesize chiral alcohols. Researchers in the lab have developed novel ADH enzymes that are highly active, stable and stereo-selective towards a number of substrates. Further, biotransformation processes have been developed that allow favourable space time yield, solute to solvent ratios and cofactor TTNs (total […]

Cyanobacteria, which constitute a quantitatively dominant phylum, are well known for their ability to carry out oxygenic photosynthesis. This prokaryotic group has been attracting attention in biofuel applications due to its favourable physiological characteristics, photosynthetic efficiency and amenability to genetic manipulations. Figure 5. Metabolic oscillations in cyanobacteria. Profiles of (A) CO2 and (B) O2 in […]