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Copyright © 2008 Jayesh Bellare; Designed by Sagar , Manu & Jaiswal. All rights reserved.
Microscopy
Optical:
Electron:
Probe:
Scattering
Flow Cytometry
Others
Microscopy
Polarizing optical microscopy employs polarized light and is especially suited for isotropic materials. The technique can be used both qualitatively and quantitatively and is an outstanding tool for materials science, geology, chemistry, biology, metallurgy and even medicine.
Key features: Olympus BX51
Key features: Olympus BX51
- Source: 12V/100W HBO (Mercury) and 75W XBO (Xenon).
- Filters: Two neutral density filters (ND6, ND25), one daylight balancing filter.
- An 8-position universal condenser, which enables brightfield, darkfield, Phase Contrast, DIC, polarization, and fluorescence with all objectives from 1.25x to 100x.
- Video attachments and film cameras features.
- Ceramic-coated stage with left or right-hand low drive control, rotating and torque adjustment mechanism.
Confocal microscopy can improve conventional fluorescence images by recording fluorescence generated from the focal plane within the sample, while rejecting all other light coming from above or below the focal plane.
Key features: Olympus IX81 FV500
Key features: Olympus IX81 FV500
- Laser Sources: Multi Argon (458 nm, 488 nm, 515 nm); He-Ne Green (543 nm); He-Ne Red (633 nm); Infra red (700-900 nm).
- Scanning Unit: Fluoview 300 (FV300); Fluoview 500 (FV500).
- Optical Modes: Single and multi-photon.
- Scanning modes: Point scanning over time; X-Y, X-Z and free line-Z; X-t and free line-t; X-Y-Z; X-Y-t and X-Z-t; X-Y-Z-t; 360° XY rotation available for all modes.
- 3D visualization: 3D animation; left/right stereo pairs; red/green stereoscopic images and cross section.
Fundamental research within the scope of cell biology, structural biology, soft matter and (bio)- nanotechnology requires investigations down to the atomic level. TEMs are of paramount importance to obtain high magnification and high resolution 2D and 3D information of cells and organelles or even smaller cell constituents.
Key features: FEI Tecnai G2 12
Key features: FEI Tecnai G2 12
- Sample size: up to 3mm diameter; 2mm in X-Y movement.
- High tilt and large field of view; Low-dose exposure.
- Beam voltage: 20kV-120kV.
- Specimen holders: Single tilt holder ±70°; Double tilt holder (x ±70°, y ±30°).;Gatan cryo-transfer holder.
- Resolution: hightest point resolution 0.3nm, line resolution 0.2nm.
High-resolution transmission electron microscope (HR-TEM) with excellent analytical performance for daily use in a multi-user environment. The configuration of the instrument combine ease-of-use with capability for routine atomic resolution imaging of crystal lattices by coherent electron scattering or phase contrast (TEM) and by incoherent electron scattering or Z-contrast in the scanning transmission electron microscopy (STEM) mode.
Key features: JEOL JEM-2100
Key features: JEOL JEM-2100
- Beam voltage : 120kV up to 200kV, Large sample tilt (=30° X- & Y-tilt).
- Cryo transfer holder: Gatan.
- Energy Dispersive X-ray Spectrometer (EDS) for the identification of different chemical species of the specimen.
- Eucentric Goniometer allows a fine shift of specimen for a wide range of magnification within areas of nanometric dimensions.
Atomic force microscope (AFM) or scanning force microscope (SFM) is a very high-resolution type of scanning probe microscope, with demonstrated resolution of fractions of a nanometer, more than 1000 times better than the optical diffraction limit. Used for visualization and measurement of surface features having nanometer sized dimensions
Key features: Nanosurf Easy Scan2
Key features: Nanosurf Easy Scan2
- Scan Head: 70 µm
- Maximum Z-range: 14 µm, Drive resolution Z: 0.21 nm
- Drive resolution XY: 1.1 nm
- XY-Linearity Mean Error: <1.2%
- Z measurement noise level (RMS, Static Mode): 0.6 nm (max. 0.8 nm)
- Z measurement noise level (RMS, Dynamic Mode): 0.04 nm (max. 0.07 nm)
Scanning tunneling microscope (STM) is a powerful technique for viewing surfaces at the atomic level.STM probes the density of states of a material using tunneling current. When a conducting tip is brought very near to a metallic or semiconducting surface, a bias between the two can allow electrons to tunnel through the vacuum between them.Uses of STM to study metals and semiconductors surface can provide non-trivial real space information.
Key features: Nanosurf Easy Scan2
Key features: Nanosurf Easy Scan2
- Scan Head 500nm: Maximum Scan range: 500nm; Maximum Z-range: 200nm; Drive resolution: 3pm.
- Spectroscopy modes: Single point measurement or multiple measurements along vector.
- Sample tilt compensation: Hardware X/Y-slope compensation.
- Scan image rotation: 0 - 360°.
SEM uses electrons to give high resolution and high depth of field and is especially suited for deciphering the surface features of the sample. Specifically, it provides information on the shape, size and arrangement of the particles making up the object that are lying on the surface of the sample or have been exposed by grinding or chemical etching; detectable features limited to a few manometers
Key features: JEOL 6400
Key features: JEOL 6400
- Resolution: SEI 35 kV, 8 mm WD; 3.5 nm
- Gun: Tungsten filament; Accelerating voltage: 5 - 20 kV
- Magnification range: 10 - 300,000 x.
- Specimen Stage: Movement x: 50 mm, y: 70 mm, z: 40 mm;Tilt: -5 - 90°; Rotation: 360°.
- Accessories: Sputter coater (Polaron); EDAX (Oxford instruments).
Light Scattering occurs when polarizable particles in a sample are bathed in the oscillating electric field of a beam of light. The short-term intensity fluctuations (dynamics) of the scattered light arise from the fact that the scattering particles are undergoing rapid thermal motions. These movements are called Brownian motion. The varying field induces oscillating dipoles in the particles and these radiate light in all directions.
Key features: Galai CIS 1
Key features: Galai CIS 1
- Scans the sample with a shaped & focused laser beam using a rotating wedge prism.
- The in-built CCD TV microscope permits in situ observatons.
- Size ranges: 0.7-150, 2-300, 5-600, 12-1200 microns.
- Sample cells: spectrophotometer cell, microscopy slide, liquid flow-through cell aerosol flow-through cell, thermo-electric cooled flow-through cell.
Scattering
In this method the dynamics of the scattered light are determined and analyzed. Typical applications of the method include Particle size distributions; Particle aggregation phenomena; Micellar systems; Micro-emulsion technology; Colloid behavior; Particle size growth; Nucleation processes & protein crystallization.
Key features: BI-200SM; Central facility
Key features: BI-200SM; Central facility
- Large angular Range: 8° to 155° with 25mm cells: 15° to 155° with 12mm cells.
- Fine Adjustment Control: Read angle to 0.01° directly on large, fine-control adjustment knob.
- Temperature Control: Heating and cooling via standard external circulator.
- Alignment Cell: Fine-screw vertical adjustment makes finding the center of rotation easier.
- Multiple Laser Line Filters: Filter wheel with 632.8 (HeNe), 514.5/488.0 nm (Ar+), one open position for weak scatterers, and 2 blank (shutter) positions.
- Additional Alignment Aperture for simpler and faster alignment of the laser.
SAXS is an analytical method to determine the structure of particle systems in terms of averaged sizes or shapes. The method is accurate, non-destructive and usually requires only a minimum of sample preparation. Applications are very broad and include the metal, cement, oil, polymer, plastics, food and pharmaceutical industries.
Key features: Anton Paar SAXSess
Key features: Anton Paar SAXSess
- X-ray source: Fine line focus, sealed tube.
- X-ray optics: 1D elliptical focusing graded multilayer mirror; Max-Flux®, Osmic Block collimator (line -shaped beam); Additional vertical slits for pinhole optics (oriented samples)
- Sample stage: External vertical and tilt alignment; Heating units -30°C-300°C
- Measuring time: 1 to 60 minutes (typical)
- Detector: Image plate; Sample – detector distance: 265 mm; Size of the image plates: 62x66 mm
- q-range: qmin: <0.077 nm-1, corresponding to a Lattice distance> 82 nm;qmax: 7 nm-1.
FACS is a celluar technique that is based on flow cytometry. It employs fluorescence detection coupled with size/granularity to sort, count and examine the cells. The fundamental element is the ability to monitor and modulate multiple parameters in a single experiment
Key features: BD FACSAria (SOS); Central facility
Key features: BD FACSAria (SOS); Central facility
- Laser Sources: 488nm (Blue); 633nm (red); 407nm (UV-Vis); 515nm (Yellow/Green) .
- Forward Scatter Detector and Filter: Photodiode detector with 488/10 bp filter;
- Side Scatter Detector and Filter: Photomultipler with 488/10 bp filter.
- Sort performancs: At 70 psi and 90 kHz with an average threshold rate of 25,000 events per second for a four-way sort results in a purity of > 98% and a yield > 80% of Poisson’s expected yield.
- Temperature control: Sample input, software-adjustable: 4, 20, 37, and 42 degrees C; Sample output, sort collection: water recirculation unit.
- Aerosol management system.
- BD FACSDiva software
Flow Cytometry
The multi-disciplinary nature of our work necessitates the use of diverse equipments and instruments. Our work draws heavily from the listed facilities, some of which are collaborative in nature.