Facilities

Sciex Triple TOF 5600 allows qualitative exploration and quantitation workflows on a single platform. The instrument provides stable mass accuracy, high sensitivity and resolution with a fast acquisition speed. The system is equipped with a nano-LC for proteomics and a UHPLC for small molecules.

Our Expertise:

• Bioprocess optimization, Fed-batch process and Data analysis.
• High cell density fermentation.
• Recombinant protein production.
• Microbial and yeast fermentation.

Applications:

• Production of therapeutic proteins and industrial enzymes.
• Small molecule production with recombinant strains.
• Detailed physiological studies of the organism with online monitoring of parameters.

Applikon Biotechnology ez-Control controller, 3 L glass vessel allows computerized control of pH, dissolved oxygen, agitation etc.  The control loops permit DO-stat and pH-stat fed-batch with cascade control.

Our Expertise:

• Quantitative analysis of 13C labelling kinetics in microbial and animal cell cultures.
• Metabolite Identification and Quantification.
• Label-based and label free proteomics.

Applications:

• 13C Metabolic flux analysis to aid metabolic engineering, drug discovery etc.
• Metabolite Identification and Quantification in ADME studies.
• Proteomics to aid drug discovery.

Lyophilizer – Labconco Corporation/ Freezer 2.5 plus, allows freeze drying of samples ranging from 0.5 ml to 500 ml.

Our Expertise:

• Freeze-drying purified proteins and crude lysates by using lyoprotectants.
• Freeze-drying whole cells.

Applications:

• Enzymes, proteins and whole cells can be preserved for future use.
• Allows temporal separation of enzyme production and characterization.
• Proteomics to aid drug discovery.

(i) Real time PCR – Agilent / Aria Mx, (ii) PCR machines, (iv) Electrophoresis units, (v) – 80 and – 20 freezers and other miscellaneous instruments for molecular biology.

Our Expertise:

• Quantitative analysis of 13C labelling kinetics in microbial and animal cell cultures.
• Metabolite Identification and Quantification.
• Label-based and label free proteomics.

Applications:

• 13C Metabolic flux analysis to aid metabolic engineering, drug discovery etc.
• Metabolite identification and quantification in ADME studies.
• Proteomics to aid drug discovery.

Stackable Incubator shakers – Eppendorf / Innova 44 R, Kuhner / LTX-C, Presens pH and DO sensors for flasks, Centrifuges, Laminar air flow and other miscellaneous instrument.

Our Expertise:

• Optimization of feeding strategies for high cell density cultivation in shake flasks.
• Online monitoring of pH and DO in shake flasks.
• Data analysis and Process optimization.
• Microbial physiology and biochemical estimation.

Applications:

• 13C Metabolic flux analysis to aid metabolic engineering, drug discovery etc.
• Measurement of kla in flasks and design of scale up based on kla.
• High titre production of enzymes and proteins in shake flasks.

Multimode plate reader- Tecan Model / infinite M200 PRO, (ii) UV – visible spectrophotometer with temperature control – Shimadzu.

Our Expertise:

• Development of enzyme assays based on UV/vis, fluorescence and luminescence measurements.
• Biochemical characterization.

Applications:

• Diagnostic assays.
• Toxicity and ADME assays.

Flat panel Photo bioreactor FMT 150 with online measurement of pH, dissolved oxygen, culture density and fluorescence quantum yield and (ii) Multicultivator – MC 1000-OD with online measurement of culture density (Both from Photon Systems Instruments, Czech Republic)

Our Expertise:

• Design of Batch, fed-batch and continuous process for phototrophic organisms.
• Bioprocess optimization and Data analysis.
• Detailed physiology and photophysiology of phototrophic organisms.
• Biofuel and small molecule production.

Applications:

• Screening and selection of photosynthetic organisms of desired properties.
• High Cell density processes.
• Effect of light, CO2 and temperature on growth of phototrophic organisms.
• 13C Metabolic flux analysis of phototrophic organisms.