Development of simulators for dielectrophoretic, electroporation and Electrofusion devices

Details

The sorting of biological cells and vesicles and characterisation for their electro-mechanical properties are important in biotechnological applications as well as developing a fundamental understanding of the response of cells and vesicles to electric fields.  Over the years, our group has developed a fair amount of understanding of response of giant vesicles and cells to electric fields, uniform and nonuniform, AC and DC fields.  This project aims to further that understanding to actual applications of continuous electric field cell devices such as electroporators, electrofusion and dielectrophoretic devices. The work will undertake simulation of actual  geometry of devices to understand the nonlinear dynamics evolving out of complex electrohyrodynamic interactions between multiple cells and fluid flow. The  work would lay the foundation for scientific design of such devices. Several new fundamental problems are expected to crop up during these investigations. The cells when subjected to electric fields can form pores which are used for electrochemotherapy. The simulations and experiments will throw light on the formation and evolution of pores, their size and spatial distribution

The project involves computations using Boundary integral method. The technical components will be Stokes flow hydrdoynamics, integral equation theory, large scale simulation

References:

1) Electric field induced pearling instability in cylindrical vesicles KP Sinha, S Gadkari, RM Thaokar Soft Matter 9 (30), 7274-7293

26 2013

2) Electrohydrodynamics of a compound vesicle under an ac electric fieldKP Sinha, RM ThaokarJournal of Physics: Condensed Matter 29 (27), 2751015 2017Conformation of charged vesicles: the Debye-Hückel and the low-curvature limit KP Sinha, RM Thaokar The European Physical Journal E 39 (7), 734 2016

3) Effect of ac electric field on the dynamics of a vesicle under shear flow in the small deformation regime KP Sinha, RM Thaokar Physical Review E 97 (3), 032404 3 2018

4) Shape deformation of a vesicle under an axisymmetric non-uniform alternating electric field KP Sinha, RM Thaokar Journal of Physics: Condensed Matter 31, 0351012 2018

5)A theoretical study on the dynamics of a compound vesicle in shear flow  KP Sinha, RM Thaokar Soft Matter 15, 6994-7017 1 2019

6) Effect of shape deformation on the dielectrophoretic force on a vesicle under axisymmetric non-uniform alternating electric field KP Sinha, RM Thaokar Journal of Electrostatics 98, 49-57 1 2019

7) Development of transmembrane potential in concentric spherical, confocal spheroidal, and bispherical vesicles subjected to nanosecond-pulse electric field S Nath, KP Sinha, RM Thaokar Physical Review E 101 (6), 062407 2020

8) Electrohydrodynamics of vesicles and capsules. KP Sinha, S Das, RB Karyappa, RM Thaokar Langmuir: the ACS Journal of Surfaces and Colloids

9) Large-deformation electrohydrodynamics of an elastic capsule in a DC electric field S Das, RM Thaokar Journal of Fluid Mechanics 841, 489-520 4 2018

10) Large deformation electrohydrodynamics of a Skalak elastic capsule in AC electric field S Das, RM Thaokar Soft Matter 14 (9), 1719-1736 3 2018

11) Deformation of a biconcave-discoid capsule in extensional flow and electric field S Das, SD Deshmukh, RM Thaokar Journal of Fluid Mechanics 860, 115-144 , 2019

Name of Faculty